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Cell Cytotoxicity Assay (CCK-8)
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The cytotoxicity of TAA and ORI on RAW264.7 and BMSCs was measured by CCK-8. More specifically, cells were planted into 96-well plates (5 × 103 cells/well) and cultured with different concentrations of TAA (0, 0.5, 1, 1.5, and 2 mg/mL) or ORI (0, 0.84, 1.69, 3.38, 6.75 and 13.5 μM) for different times. Then, DMEM supplemented with CCK-8 solution was added to each well for continuous culture for 2 h. Spectrophotometer was used to detect the absorbance at 450 nm.
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