Rotarod Test

Passive motor coordination function was tested by using a Harvard Instruments accelerating rotarod. To achieve a steady performance, all animals were pre-trained for two days. On day 1, the mice were trained three times on a spinning rod that advanced from 2 to 20 revolutions per min (rpm) in 300 s. On day 2, the mice were trained on a rod that was accelerated at 300 s from 3 rpm to 30 rpm twice and 4 rpm to 40 rpm once. The test began on the third day by using a revolving rod that was advanced from 4 to 40 rpm in 300 s. The duration of the stay on the rod was measured. An average length of three repeated tests for each animal was used for data analysis. GraphPad (GraphPad Software Inc., San Diego, CA, USA) was used for statistical analysis. For multiple comparisons, a two-way analysis of variance (ANOVA) followed by Bonferroni's post-hoc test was used.