Histopathology assessments were performed by four board-certified clinical pathologists independently to improve rigor and reproducibility. All H&E slides were assessed in a CLIA certified pathology laboratory using an inverted brightfield microscope (Olympus BX43). Image analysis was performed using the HALO image analysis software (Indica labs). Assessments were made based on the spatial clustering produced by HDR-SC and histopathology assessment from an immediate adjacent section 4μm apart.
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