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NIH-3T3 cells were transfected with methylated or un-methylated Wt or mutagenized pCpG-Ankrd26 vector and Renilla control vector (Promega) by lipofectamine (Life Technologies), following manufacturer’s instructions. Where indicated, cells were co-transfected with pCl.p300 expression vector (Promega). Firefly luciferase activity of each transfection was normalized for transfection efficiency against Renilla luciferase activity.
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