2.1. Microorganisms and Culture Conditions

B. velezensis HMB26553 was regularly cultivated at 37 °C on the Luria Bertani medium. Strain HMB26553 was cultured in Landy broth at 30 °C and 180 rpm to produce lipopeptides and other secondary metabolites [21]. The antifungal activity test was conducted on the phytopathogen R. solani using a modified version of Su et al.’s approach [22]. In short, a 9-cm potato dextrose agar (PDA) plate containing a 6-mm-diameter disc of R. solani was infected 2 cm from the center with B. velezensis HMB26553 using a sterilized toothpick. After a 3-day incubation at 25 °C, the diameter of the inhibitory zone was lastly measured.