Measurement of tumor necrosis factor-α, interleukin-1β, interleukin-6, and myeloperoxidase contents

OC Ouyang Chen
ZC Zhiyong Cao
HL He Li
ZY Zhouheng Ye
RZ Rongjia Zhang
NZ Ning Zhang
JH Junlong Huang
TZ Ting Zhang
LW Liping Wang
LH Ling Han
WL Wenwu Liu
XS Xuejun Sun
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Heart supernatants were collected and used to measure the contents of TNF-α, IL-1β, and MPO using commercial enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems, Minneapolis, MN, USA), following the manufacturer’s instructions. The absorbance was measured at 450 nm using a microplate reader (ELx800; BioTek Instruments, Winooski, VT, USA) and the contents of these proteins were calculated using standard curves.

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