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For luciferase assays, cells were grown in 96-well, white tissue-culture plates (Corning). After exposure to SPIKE particles as indicated, cells were lysed with Nano-Glo Luciferase Assay reagent (Promega). Luciferase reagent was prepared according to the manufacturer’s protocol. For lysis, 100 μl of a 1:1 mix of luciferase reagent and cell culture media was added to each well of the 96-well plate and incubated at room temperature for 3 minutes. The plate was read using a Biotek Synergy H1 multimode plate reader.
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