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Acquisition of MALDI-TOF mass spectra and data analysis

GL Gerald Larrouy-Maumus
LD Laurent Dortet
IN Ilka D. Nix
TM Thomas Maier
BO Boris Oberheitmann
KS Katrin Sparbier
MK Markus Kostrzewa
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The spectra were recorded in the linear negative-ion mode (laser intensity 45%, ion source 1 = 15.00 kV) of the MALDI Biotyper® sirius system. Each spectrum corresponded to ion accumulation of 200–1000 laser shots randomly distributed on the spot.

Spectra acquisition and simultaneous evaluation were done automatically in the MBT Compass HT using the MBT HT LipidART Module. A “log[SN](R)” value is calculated by the software. The log [SN](R) contains two important information of the lipid A mass spectra. The first value is an indicator of the peak intensities/signal-to-noise values (SN) of detected lipid A molecules. The second value shows the ratio of the lipid A modification to the native lipid A peak. The log [SN](R) value shall be used as an indicator of the general spectra quality and the height of the modified lipid A peaks compared to the native lipid A peak.

For visual inspection, the spectra obtained were processed with default parameters using FlexAnalysis v.3.4 software (Bruker Daltonics, Germany).

Performance evaluation was done according to DIN EN ISO 20776–2:2021 [21].

Copyright and License information: The Author(s) ©2023
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