RNA extraction

IP Samples were thawed on ice and centrifuged to remove any residual beads. Genomic DNA were removed from the supernatants using columns from the Qiagen RNeasy Plus Micro kit per manufacturer’s instructions. For final elution, 14 μl of RNase-free water were added. RNA samples were stored at −80°C before quantification and use for reverse transcription (ABI High-Capacity cDNA Reverse Transcription Kit).