The cDNA sequence encoding mouse high- and low-MW Gab1 was subcloned into the shuttle plasmid pACCMVpLpA. Recombinant adenovirus was then obtained according to the homologous recombination system described previously [32,37]. The adenovirus vector expressing β-gal was used as a control.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.