After arrival, goats were allowed to acclimate for at least 72 h before two surgical procedures, each separated by 2 wk. After 2 wk of recovery from the second surgery, the goats were studied in the awake, sleep, and anesthetized state for 4-6 wk thereafter. At the completion of the protocol, goats were euthanized and the brain tissue was extracted for histological confirmation of microtubule (MT) placement.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.