Strain identification

Chromosomal DNAs were isolated using a commercial kit and protocol (A&A Biotechnology, Poland). Polymerase chain reactions (PCRs) were performed with PrimeStar HS DNA Polymerase (TaKaRa) under standard conditions. The amplified 16S rRNA gene was obtained from isolate by PCR with the universal primers F27 (5′-AGAGTTTGATCMTGGCTCAG-3′) and R1492 (5′-TACGGYTACCTTGTTACGACTT-3′) [9] which are targeted to universally conserved regions and permit the amplification of an approximately 1500-bp fragment. Oligonucleotide synthesis and DNA sequencing were performed by Genomed S.A., Warsaw, Poland. The nucleotide sequences were analysed using BLAST against the nucleotide database on the NCBI website. The analysis revealed the highest identity, i.e., 99%, to the nucleotide sequence of the 16S rDNA gene of E. faecalis JF85 (GeneBank {"type":"entrez-nucleotide","attrs":{"text":"KT343158.1","term_id":"972987927","term_text":"KT343158.1"}}KT343158.1), thus identifying the taxonomic position of the strain as E. faecalis.