2.2.2. Large scale production process

Three separate batches of SPC were produced from LFSF1 by following the previously described production process with some modifications. For making each batch, 350 g of LFSF1 and 3.5 l of distilled water were mixed using a mixer (KitchenAid®; Benton Harbor, MI, United States) for 30 min at 22°C. After the washing process with water, the mixture was transferred into a 5 l plastic container, and it was covered with aluminum foil. For solid–liquid separation, the mixture was first left for settlement for 2 h at room temperature. At the end of 2 h, the precipitate was separated by using a woven 200-micron commercial-grade nut-milk bag (Nut Milk Bag; Vandoona LLC., NY, United States). The wet protein concentrate collected in the bag was then uniformly spread onto a pre-weighed metal drying tray and dried by using a dehydrator (Excalibur Dehydrators®, Sacramento, CA, United States) at 60°C for about 4 h until its moisture content decreased below 15%. The dried SPC was then ground into a powder (to pass 150 μm openings) using a hammer mill (Lab Mill 3,100, PerkinElmer Inc.; Waltham, MA, United States). Dried SPC materials were placed in sealed bags and kept at −20°C until further analysis.