Assessment of acetylcholinesterase activity in the hippocampus and the frontal cortex

The activity of acetylcholinesterase was evaluated using acetylcholine iodide as a substrate and 5,5'-dithiobis-2 nitro benzoic acid (DTNB) as the coloring material (39). Phosphate buffer was present in 1.0 ml of the reaction mixture (0.1 M, pH 7.4). DTNB and reacted combination was added 0.1 mL of acetylthiocholine iodide. Subsequently, the wavelength of the absorbance was measured using a spectrophotometer (412 nm) (10 min), the rate of breakdown of acetylcholine iodide, were determined, and the results were represented in μmoles/min/mg protein.