3.3.6. DAPI Staining

The ability of substituted SOX (4a) to prompt apoptosis in PC-3 cells was done through DAPI staining [83]. The PC-3 cells were challenged with varying doses of 4a (0, 6.25, 12.5, 25, 50, and 100 μM and incubated for 24 h at 37 °C and then stained with DAPI. DAPI-specific fluorescence in 4a-treated and untreated cells was captured using a blue filter in a FLoid Imaging station (Thermo-Scientific, Waltham, MA, USA).