2.7. Statistical Analysis

Based on the above pipeline, we obtained the allelic genotype, AF, and forensic statistical parameters of 50 MHs among 137 Southwest Chinese Han individuals, including homozygosity (Hom), heterozygosity (Het), match probability (MP), discrimination power (DP), probability of exclusion (PE), polymorphism information content (PIC), and the typical paternity index (TPI) by using the Modified-Powerstates v. 1.2 (Promega, Madison, WI, USA) [36]. Then we used the following formula to calculate combined match probability (CMP), combined discrimination power (CDP), and combined probability of exclusion (CPE), respectively, including CMP = 1 − ΣP(1 − MP₁) (1 − MP₂) (1 − MP₃) … (1 − MP₅₀), CDP = 1 − ΣP(1 − DP₁) (1 − DP₂) (1 − DP₃) … (1 − DP₅₀) and CPE = 1 − ΣP(1 − PE₁) (1 − PE₂) (1 − PE₃) … (1 − PE₅₀), where 1 … 50 represent the 50 MHs. The A_e value was calculated as the reciprocal of homozygosity: 1/∑p_i², where p_i is the frequency of allele i and summation includes all alleles at the MH. In addition, the Hardy–Weinberg equilibrium (HWE) p-value and LD value were calculated using Arlequin v3.5 (University of Berne, Lausanne, Switzerland) [18,37].