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Tandem mRFP-GFP-LC3 fluorescence microscopy
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Cells were transfected with adenovirus expressing mRFP-GFP-LC3 (Hanbio, Shanghai, China) at 10 MOI for 24 h, and then treated with or without other agentia. The cells were analyzed by confocal laser scanning microscopy (LSM510; Carl Zeiss AB, Jena, Germany).
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