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DNA methylation

AZ Alberto Zenere
SH Sandra Hellberg
GL Georgia Papapavlou Lingehed
MS Maria Svenvik
JM Johan Mellergård
CD Charlotte Dahle
MV Magnus Vrethem
JR Johanna Raffetseder
MK Mohsen Khademi
TO Tomas Olsson
MB Marie Blomberg
MJ Maria C. Jenmalm
CA Claudio Altafini
MG Mika Gustafsson
JE Jan Ernerudh
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DNA from 154 samples (from 78 resting CD4+ and 76 resting CD8+ T cells, Additional file 2: Table S1 and Additional file 1: Fig. S3) was sent to the SNP&SEQ-technology platform at SciLifeLab (Uppsala University, Uppsala, Sweden). Only 6 samples were available BP in MS and due to insufficient amount of material, samples were also missing for two time points in CD8+ in MS (Additional file 2: Table S1 and Additional file 1: Fig. S3). Bisulfite conversion was performed using the EZ DNA Methylation™ Kit (Zymo Research) with 250 ng of DNA per sample as input. The bisulfite converted DNA was eluted in 15 μl Elution Buffer according to the manufacturer´s protocol, evaporated to a volume of < 4 μl, and used for methylation analysis using Infinium Human MethylationEPIC BeadChip array (Illumina) covering 850,000 methylation sites across the genome.

Copyright and License information: The Author(s) ©2023
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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