Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
CryoEM grids were prepared applying three μL of each sample to holey carbon grids (Quantifoil, 1.2/1.3), incubated briefly in the chamber at 4 C with 100% humidity, and vitrified in liquid ethane using a Vitrobot IV (Thermo Fisher Scientific). Grids were then transferred to a Titan Krios microscope, operated at 300 keV, equipped with Falcon III detector for data collection. In total, 3276 movies (SPFMV) and 2260 movies (SPMMV) were collected, each movie containing 32 frames with a dose of ~1e-/A2 per second.
Copyright and License information: The Author(s) ©2023
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
