Glutamate assay

We added 1 mM Glutamate solution to each well for a final concentration of 100 uM, and the cells were incubated at 37°C for 10 min, which is known to be enough to induce a loss of drebrin clusters without affecting cell viability in rat hippocampal neuron cultures.¹⁷ We added the same volume of water was added for the “0 μm” control wells. At the end of the 10 min incubation, we fixed the cells immediately with 4% paraformaldehyde in 0.1 M phosphate buffer to proceed with immunocytochemistry.