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TS Takashi Suto
DK Daiki Kato
IK Ikuya Koibuchi
YA Yuki Arai
JO Jo Ohta
TH Tadanao Hiroki
HO Hideaki Obata
SS Shigeru Saito
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Spontaneously hypertensive rats (SHR/Izm, n = 144) and their normotensive controls (Wister Kyoto: WKY/Izm, n = 123) were supplied by the Disease Model Cooperative Research Association (Kyoto, Japan). Adult male and female rats, 6 to 7 weeks old (210–240 g) at the time of paw incision surgery, were housed under a 12-h light–dark cycle, and fed ad libitum. The animals were randomly assigned to each experimental group (SHR, SHR-ATX, and control). In the SHR-ATX group, 5 weeks old SHR were injected with atomoxetine (0.3 mg/kg) intraperitoneally once a day for 14 days. Atomoxetine hydrochloride (ATX) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan) and was dissolved in saline (0.3 mg/ml). The following experiments were performed at an approximately 24-h interval from the last atomoxetine injection.

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