Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Collection of tissue slices
Ask a
question
Favorite
The rats were deeply anesthetized with ketamine and perfused transcardially with 100 mL normal saline (0.9%). Liver, kidney, and lung specimens were fixed in 4% buffered formalin and embedded in paraffin. Sections (14 µm) from paraffin-embedded tissue blocks were cut using a microtome (RM2125 rotary; Leica Microsystems, Wetzlar, Germany) and collected on silane-coated slides (Muto Pure Chemical, Tokyo, Japan) for histology and immunohistochemistry and stored at −70°C.
Copyright and License information: ©2017 Bahar et al. This work is published and licensed by Dove Medical Press Limited
The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
