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5.4.2. Measuring the Maximum Photochemical Yield of Photosystem II (Fv/Fm)

HF Hind Emad Fadoul
LA Lucas J. Albano
MB Matthew E. Bergman
MP Michael A. Phillips
MJ Marc T. J. Johnson
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To assess the effect of freezing on the photosynthetic activity of white clover, analysis of photosystem II Fv/Fm was measured during the experiment. We measured photosynthetic activity at each of the five time points noted above from a single green leaflet on each whole plant. All plants were dark-acclimated followed by measuring the maximum photochemical yield of photosystem II (Fv/Fm) using a photosynthesis yield analyzer JUNIOR-PAM (Walz Teaching-PAM fluorometer (PAM-200), Heinz Walz GmbH, Effeltrich, Germany) and WinControl-3 software. Three independent measurements were averaged for each plant per time point.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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