2.4. Mitogenomic Comparison of Six Ophiocordyceps Species

DNASTAR laserene 7.1, an important bioinformatics tool, was used to evaluate the nucleotide base composition of six mitogenomes (http://www.dnastar.com/t-dnastar-lasergene.aspx) (accessed on 1 April 2022). Comparison in terms of nucleotide length, as well as the CU encoding information of RNAs of the complete mitogenomes of O. lanpingensis, O. sinensis, Hirsutella vermicola (H. vermicola), H. thompsonii, H. rhossiliensis, and H. minnesotensis, was computed using MEGA 6.0 software [24]. Differences in the G-nucleotide base composition and C-nucleotide base composition on the strand disproportionateness were determined using GC and AT skew calculations. These important replication phenomena, wherein prokaryotes present a relative excess of nucleotides, were calculated using the following equations:

The occurrence of CU was noted using Sequence Manipulation Suite sequence estimating software. The overall mean genetic distances between 15 core protein-coding genes (PCGs) of 6 species were estimated with respect to the Kimura-2-parameter (K2P) substitution model in MEGA 6.0 and using GC [24]. Finally, the synteny of six mitogenomes was performed using the multiple genome alignment application Mauve v2.4.0 [23].