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2.8. Measurement of surface receptor expression

DB David B. Bartlett
OF Oliver Fox
CM Clare L. McNulty
HG Hannah L. Greenwood
LM Laura Murphy
ES Elizabeth Sapey
MG Martin Goodman
NC Nicola Crabtree
CT Cecilie Thøgersen-Ntoumani
JF James P. Fisher
AW Anton J.M. Wagenmakers
JL Janet M. Lord
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Freshly isolated neutrophils (1 × 105 ml−1) were stained with anti-CXCR1-FITC (eBioscience, Hatfield, UK, clone 8F1-1-4), anti-CXCR2-PE (eBioscience, clone 5E8-C7-F10), anti-CD16-APC (eBioscience, clone CB16), anti-CD16-FITC (BD Bioscience, clone 3G8), anti-CD11b-APC (BD Bioscience, clone ICRF44), anti-CD18-PE (BD Bioscience, clone 6.7) or their relevant concentration-matched isotype control for 30 min on ice in the dark. Following incubation, cells were washed in PBS/1% BSA and resuspended in PBS/1% BSA for analysis by flow cytometry. 10,000 CD16+ neutrophils were acquired for analysis on a CyAnADP™ 430 flow cytometer and data analysed using Summit v4.3 software.

Copyright and License information:  ©2016 The Authors
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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