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Lentiviral particles PLKO.1 and PLKO.1-shSTC1 were provided by the Beijing Institute of Radiation Medicine. Viruses were packaged by co-transfection with PLKO.1 and PLKO.1-shSTC1 into 293T cells. The supernatants containing viruses were collected 48 hr after transfection, then the centrifuged and resuspended lentivirus were used for further transduction of hMSCs in Opti-MEM. The stable STC1 knockdown hMSCs were obtained after 7–10 days of puromycin selection in 96-well plates. Transduction efficiency was determined by fluorescent microscopy.
Copyright and License information: ©2023, Zhang, Liu et al
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