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Small intestinal crypt cells were isolated as described above at 20, 24, 36, 48, and 50 h post tamoxifen induction. RNAs were extracted using TRIzol reagent according to standard protocols. Real-time PCR reactions were performed blindly in triplicate or duplicate using SYBR green master mix. PCR primers used are listed in Supplementary Table S2.
Copyright and License information: ©2023 The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
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