Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
HeLa WT and RAGE−/− cells were either left untreated or stimulated with hydroxyurea (HU) or glyoxal for 4 h. About 500 cells per well in six-well plates were seeded and incubated for colony formation. After 10 days, colonies were stained with 0.5% Crystal Violet (Sigma) for 20 min. The colonies were counted with OpenCFU (colony counting software). The percentage survival was calculated by normalizing the data with untreated (100%) (RAGE+/+ or RAGE−/−) cells.
Copyright and License information: ©2023 The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact moc.puo@snoissimrep.slanruoj
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
