Flow cytometry and FACS

HZ Han Zou
BP Bradley Poore
EB Emily E. Brown
JQ Jieqi Qian
BX Bin Xie
EA Evridiki Asimakidou
VR Vladislav Razskazovskiy
DA Deanna Ayrapetian
VS Vaibhav Sharma
SX Shunjin Xia
FL Fei Liu
AC Apeng Chen
YG Yongchang Guan
ZL Zhengwei Li
SW Siyi Wanggou
OS Olivier Saulnier
ML Michelle Ly
WF Wendy Fellows-Mayle
GX Guifa Xi
TT Tadanori Tomita
AR Adam C. Resnick
SM Stephen C. Mack
ER Eric H. Raabe
CE Charles G. Eberhart
DS Dandan Sun
BS Beth E. Stronach
SA Sameer Agnihotri
GK Gary Kohanbash
SL Songjian Lu
KH Karl Herrup
JR Jeremy N. Rich
GG George K. Gittes
AB Alberto Broniscer
ZH Zhongliang Hu
XL Xuejun Li
IP Ian F. Pollack
RF Robert M. Friedlander
SH Sarah J. Hainer
MT Michael D. Taylor
BH Baoli Hu
request Request a Protocol
ask Ask a question
Favorite

Mice showing neurological signs of late-stage brain tumours or deemed end point were killed, and blood was collected through cardiac exsanguination under deep general anaesthesia. The collected blood (500–900 µl) was swiftly prepared for flow cytometry using RBC lysis buffer (Invitrogen, 00-4333), and cells were suspended in ice-cold PBS with 1% BSA and 2 mM EDTA. After incubation with propidium iodide (Thermo Fisher, P3566) in the dark, the stained cells were analysed using a BD Fortessa analyser. FACS was performed using a BD FACSAria cell sorter. Data were analysed using FlowJo software (v.10.6.1).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A