Differential scanning calorimetry (DSC)

NW Nina E. Weisser
MS Mario Sanches
EE Eric Escobar-Cabrera
JO Jason O’Toole
EW Elizabeth Whalen
PC Peter W. Y. Chan
GW Grant Wickman
LA Libin Abraham
KC Kate Choi
BH Bryant Harbourne
AS Antonios Samiotakis
AR Andrea Hernández Rojas
GV Gesa Volkers
JW Jodi Wong
CA Claire E. Atkinson
JB Jason Baardsnes
LW Liam J. Worrall
DB Duncan Browman
ES Emma E. Smith
PB Priya Baichoo
CC Chi Wing Cheng
JG Joy Guedia
SK Sohyeong Kang
AM Abhishek Mukhopadhyay
LN Lisa Newhook
AO Anders Ohrn
PR Prajwal Raghunatha
MZ Matteo Zago-Schmitt
JS Joseph D. Schrag
JS Joel Smith
PZ Patricia Zwierzchowski
JS Joshua M. Scurll
VF Vincent Fung
SB Sonia Black
NS Natalie C. J. Strynadka
MG Michael R. Gold
LP Leonard G. Presta
GN Gordon Ng
SD Surjit Dixit
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Antibody thermal stability was measured using DSC as follows: Ab samples in PBS were used for DSC analysis with a VP-Capillary DSC (GE Healthcare, Chicago, IL). At the start of each DSC run, 5 buffer blank injections were performed to stabilize the baseline, and a buffer injection was placed before each sample injection for referencing. Each sample was scanned from 20 to 100 °C at a 60 °C/h rate, with low feedback, 8 s filter, 3 or 5 min pre-scan thermostat, and 70 psi nitrogen pressure. The resulting thermograms were referenced and analyzed using Origin 7 software (OriginLab Corporation, Northampton, MA) to determine melting temperature (Tm) as an indicator of thermal stability.

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