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Potential inhibition of the proteolytic activity of MdpL was studied with a panel of protease inhibitors: AEBSF, ALLN, antipain, bestatin, chymostatin, E64, EDTA-Na2, leupeptin, pepstatin, phosphoramidon, and PMSF (G-Biosciences, Geno Technology Inc., United States) following the manufacturer’s instruction. MdpL inhibition was analyzed by gel electrophoresis using Etanercept as the substrate.
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