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To determine the level of late-stage apoptosis, CMECs grown on coverslips were fixed with 4% paraformaldehyde at RT for 30 min. By using a terminal deoxynucleotidyl transferase mediated dUTP-nick end labeling (TUNEL) detection kit (Vazyme, Nanjing, Jiangsu, China) according to the manufacturer's instruction, the images of TUNEL-positive/4′,6-diamidino-2-phenylindole (DAPI)-stained cells were observed using a confocal microscope (Zeiss, Oberkochen, Germany).
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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