Statistics and reproducibility

The data reproducibility was confirmed by at least three experiments. Individual data points were shown as dots in the graph. The statistical significance of differences between two groups was evaluated using a two-tailed paired or unpaired t-test. Comparisons among three or more groups were performed using ordinary or repeated measures one-way ANOVA with multiple comparison test. When indicated, the log-transformed values were used for comparison. We added 1 to the concentration values of cytokines before log transformation to account for zero values (Fig. 3n, o). Correlation between CFSE dilution and the frequency of CCR7⁺ cells (Fig. 1e, f) was estimated by Pearson correlation analysis. The frequency of CD83⁺ or CD83⁺CCR7⁺ cells was compared among the groups by Fisher’s exact test with multiple testing correction (Fig. 7d). We used a P-value of 0.05 as a threshold to determine statistical significance. All statistical analyses were performed using GraphPad Prism 9 software or R 4.0.5. No statistical method was used to determine the sample size.