In order to explore the pathways associated with risk signatures involved in ES, Gene set “h.all.v7.5.1.symbols.gmt” from KEGG annotation online were downloaded, then GSEA was conducted [25]. For further understanding the role of CRGs, ROC curves via “pROC” package were used to evaluate the diagnostic ability of signatures in ES occurrence, which was validated in GSE68776.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.