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Total mRNA was extracted from renal tissues of WT-IR and KO-IR groups (n = 3) using TRIzol. The RNA quality was verified using a 2100 Bioanalyzer and ND-2000, and the samples were sequenced by Illumina HiSEQ 4000 of Shanghai Magi Biomedical Technology Co., Ltd. All differentially expressed genes were used for heat maps analysis and KEGG enrichment analysis. For KEGG enrichment analysis, P < 0.05 was considered statistically significant.
Copyright and License information: ©2023 Yiwen Chen et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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