2.7. HPLC/MS

The goal of this study was to quantify the amount of SA, JA, and ET accumulated in this way; seeds and seedlings were handled as described previously. Seedlings’ aerobatic sections were collected for SA, JA, and ET sampling [32]. This was done using an HPLC/MS (Shimazu Research Instruments, ODS-C18, 3 m, 2.1 per 150 mm, Kyoto, Japan). Methanol mobile phase, 60 percent, and 4 °C sample temperature were used in HPLC. Sim system in negative ion mode was used with the following parameters: solvent 250 °C, heat block 200 °C, gas flow rates 10 L/min, nebulizing gas 1.5 L/min, detector voltage 1.30 kV, and interface 3 kV (SA m/z: 137.00; JA: 209.05).