3.7.1. Preparation of Spleen Cells from Mice

Female BALB/c mice were sacrificed by cervical dislocation, and the spleens were removed aseptically. Mononuclear cell suspensions were prepared after cell debris and clumps were removed. Erythrocytes were depleted with ammonium chloride buffer solution. Lymphocytes were washed and resuspended in RPMI-1640 medium supplemented with 10% FBS, penicillin (100 U/mL), and streptomycin (100 mg/mL).