Cell Culture and AuNS@PEG Nanoparticle Exposure

Neuroglia cells were collected from rat spinal cord tissues. The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, USA) supplemented with 10% fetal bovine serum, 100 U per ml penicillin, and 100 μg per ml streptomycin at 37 °C in a humidified incubator with 5% CO₂. Cells were seeded in culture plates followed by exposure to AuNS@PEG nanoparticles for 2 h at certain concentrations (50, 100, 200, 500, and 1000 ppm). DMEM without AuNS@PEG nanoparticles were used as the control group.