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Immune score, stromal score, and estimate score between two risk groups were calculated using R “estimate” package (R version 4.0.2). A total of 52 genes were selected to be immune-checkpoint-relevant transcripts, and the expression values of these 52 genes were extracted to perform differential expression analysis between two risk groups. Furthermore, correlation analysis between risk score and expression of CTLA4, PD1, PD-L1, and PD-L2 was performed using Pearson’s correlation.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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