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Five grams of each fecal specimen was placed into two 15ml falcon tubes; one tube contained 10 ml phosphate-buffered saline (PBS) and the other 10 ml isopropanol. Each tube was vortexed and sieved through cotton gauze to remove large particles. The mixture in the isopropanol was stored in a refrigerator (4°C) for use in molecular analysis. The sample in PBS was centrifuged at 10,000 rpm for 5 minutes, and the supernatant was used for the microscopy [49].
Copyright and License information: ©2023 Mensah et al
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