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3.1. Step-by-step protocol: Isolation of mouse renal peritubular endothelial cells (MRPEC)

AT Austin D. Thompson
JJ Jaroslav Janda
RS Rick G. Schnellmann
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° (∧∧Over/under digestion of the tissue pieces may result in a reduced cellular yield and/or viability; digestion times should be experimentally adjusted by the researcher accordingly, to ensure over/under digestion does not occur).

° Note, cells of interest will be contained within the column flow through (the negative fraction) in this step. Cells that are magnetically labeled in this step, and thus retained within the LS column (the positive fraction), contain CD326+ epithelial cells that might reduce the overall purity of isolated MRPECs and are subsequently discarded.

A graphical overview of the step-by-step procedure for the isolation and monoculture of primary MRPECs is shown in Figure 1.

A graphical overview of the step-by-step procedure for the isolation and monoculture of primary MRPECs.

Copyright and License information:  ©2023 Thompson, Janda and Schnellmann.
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