Isolation of active compound from {"type":"entrez-nucleotide","attrs":{"text":"EL001672","term_id":"124923481","term_text":"EL001672"}}EL001672

MV Mücahit Varlı
EL Eun-Young Lee
YY Yi Yang
RZ Rui Zhou
İT İsa Taş
SP Sultan Pulat
CG Chathurika D. B. Gamage
SP So-Yeon Park
JH Jae-Seoun Hur
SN Sang-Jip Nam
HK Hangun Kim
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The crude extract of EL001672 (62 g) was fractionated by the flash column chromatography on silica gel eluted with CH2Cl2/CH3OH (100/0, 100/2, 100/1, 100/5, 100/10, 100/20, 100/50, 100/100 and 0/100, v/v, each of 600 mL) to obtain eight fractions (Fraction 1 to Fraction 8). The fraction two (7.88 g) was further fractionated by the flash column chromatography on C-18 resin eluted with H2O/CH3OH (80/20, 60/40, 50/50, 40/60, 30/70, 20/80, 0/100, each of 600 mL) to obtain eight subfractions (Fraction 1 to Fraction 8). The subfraction seven was isolated by reversed-phase HPLC (Phenomenex Luna C-18 (2), 250 × 100 mm, 2.0 mL/min, 5 μm, 100 Å, UV = 280 nm) using an isocratic condition 85% CH3CN in H2O to obtain 1′-O-methyl-averantin (1.4 mg, tR = 22.5 min).

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