4.6.1 Single worm PCR and Sanger Sequencing

Individual worms were picked and transferred into tubes with 10 µL H₂O or M9 buffer. Tubes were placed at −80°C for at least 1 h 10 µL 2x worm lysis buffer (Supplementary Table S2) was added, incubated at 55°C for 3 h and heated at 95°C for 10 min. We designed primers to amplify the genomic DNA fragment containing the cut site. PCR products were resolved on a 0.8% agarose gel. Sanger sequencing was performed through Eurofins Scientific SE. Sequences were analyzed using the ICE CRISPR analysis tool v3 (https://www.synthego.com; Conant et al., 2022) and Geneious Pro 5.5.6 (Kearse et al., 2012). List of primers used for this study can be found on Supplementary Table S5.