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Optical identification of locomotor command regions

LH Li-Ju Hsu
MB Maëlle Bertho
OK Ole Kiehn
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To test for the involvement of glutamatergic and/or serotonergic neurons in the locomotor command signal, in similar ways as electrical stimulation, we performed, an entire transverse cut for effective locomotor initiating sites using optical stimulation in crosses of Vglut2Cre and SERTCre and R26RChR2-EYFP mice. For stimulation, we used a 473 nm laser system (UGA-40; Rapp Optoelectronic), which delivered blue light at an intensity of 30 mW/mm2 105. The blue light was directed at the preparation using an optical fiber (200 μm core, 0.22 NA, Thorlabs). The illumination was carried out continuously for 15–20 s. Two to four trials were tested for each preparation.

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