Anesthesia

LL Luocheng Lv
WG Weimin Guo
WG Wei Guan
YC Yilin Chen
RH Ruijin Huang
ZY Ziqiang Yuan
QP Qin Pu
SF Shanshan Feng
XZ Xin Zheng
YL Yanmei Li
LX Luanjuan Xiao
HZ Hui Zhao
XQ Xufeng Qi
DC Dongqing Cai
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Animals were maintained at room temperature (26 to 28 °C) and anesthetized with tricaine mesylate (MS-222; 1 mg/mL; TCI, Shanghai, China). X. tropicalis was transferred into a 500 mL bath containing Milli-Q water with 1 mg/mL tricaine mesylate. X. tropicalis was identified as being fully anesthetized when there was no movement and no response to touch. This process took approximately 3 to 5 min.

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