Animals were maintained at room temperature (26 to 28 °C) and anesthetized with tricaine mesylate (MS-222; 1 mg/mL; TCI, Shanghai, China). X. tropicalis was transferred into a 500 mL bath containing Milli-Q water with 1 mg/mL tricaine mesylate. X. tropicalis was identified as being fully anesthetized when there was no movement and no response to touch. This process took approximately 3 to 5 min.
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