Gas chromatography-thermal conductivity detector (GC-TCD) analysis

SU Sakiko Ueda
YK Yuhki Kawamura
HI Hiroko Iijima
MN Mitsuharu Nakajima
TS Tomokazu Shirai
MO Mami Okamoto
AK Akihiko Kondo
MH Masami Yokota Hirai
TO Takashi Osanai
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Cells were incubated under dark, anaerobic conditions as wells as the experiment of organic acid production. The accumulated H2 gas in the headspace of a GC-vial was measured with a gas chromatograph (GC-2010 Plus AT, Shimadzu, Kyoto, Japan) as described previously24 and in accordance with the manufacturer’s instructions. N2 was used as the carrier gas with a flow rate of 10 mL/min.

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