To evaluate the photothermal property, the Bi-BG scaffolds were placed in a 48-well culture plate, irradiated by the 808 nm NIR laser (LDT-808, Shanghai Connect Fiber Optics Company) for 5 min, and the temperature variation was imaged and recorded in real time by an infrared thermal imaging system (223S, Fotirc). Different power density of the light was applied, including low (1 W/cm2), medium (2 W/cm2), and high (3 W/cm2). The photothermal effect of the scaffolds in wet condition (PBS) was also evaluated for 10 min. Finally, the photothermal conversion curve over time was plotted using FLIR R&D software.
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