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Collection of human diploid embryos
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The donated IVM oocytes were in vitro fertilized in G-IVFTM PLUS medium (Vitrolife, 10136) in a humidified atmosphere at 37 °C with 6% CO2 in air. Cultured 4-cell, 8-cell embryos, morula and morphological AA grade blastocysts were collected around 48 h, 3 days, 4 days and 5 days after fertilization, respectively. Only high-quality embryos were selected and collected for further experiments.
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