Ear and colon tissue were fixed in 4% paraformaldehyde in PBS, embedded in paraffin, sectioned, and stained with H&E for histopathologic examination. Images were captured using an Olympus BX600 microscope (Olympus Corporation, Tokyo, Japan) and a SPOT Flex camera (Olympus Corporation, Tokyo, Japan), and were analyzed with ImagePro Plus (version 6.0, Media Cybernetics) software. Epithelial thickness and cell infiltration were evaluated in independent regions. A pathological score based on the Baker score system was obtained to assess the severity of psoriasiform skin inflammation in ear tissues.
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