4.2. Cell Culture

AS Andrea Sánchez-Medina
MR Mónica Redondo-Puente
RD Rudolf Dupak
LB Laura Bravo-Clemente
LG Luis Goya
BS Beatriz Sarriá
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Human hepatic HepG2 cells were maintained in a humidified incubator containing 5% CO2 and 95% air at 37 °C. They were grown in DMEM F-12 medium and supplemented with 2.5% FBS and 50 mg/L each of gentamicin, penicillin, and streptomycin.

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